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GLUT5 antibody [14C8]

Overexpression
Anti-GLUT5 antibody [14C8] used in Flow cytometry (FACS). GTX83627
Anti-GLUT5 antibody [14C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83627
Anti-GLUT5 antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX83627
Anti-GLUT5 antibody [14C8] used in Western Blot (WB). GTX83627
Anti-GLUT5 antibody [14C8] used in Western Blot (WB). GTX83627
Anti-GLUT5 antibody [14C8] used in Immunoprecipitation (IP). GTX83627

Cat. No. GTX83627

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 14C8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all GLUT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 2 member 5 , GLUT-5 , GLUT5

Cellular Localization

Apical cell membrane,Cell membrane

Background

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

Database

Research Area

DATA IMAGES

Anti-GLUT5 antibody [14C8] used in Flow cytometry (FACS). GTX83627

GTX83627 FACS Image

FACS analysis of HEK293T cells transfected with either GLUT5 plasmid(Red) or empty vector control plasmid(Blue) using GTX83627 GLUT5 antibody [14C8].

Anti-GLUT5 antibody [14C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83627

GTX83627 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GLUT5 plasmid using GTX83627 GLUT5 antibody [14C8].

Anti-GLUT5 antibody [14C8] used in IHC (Paraffin sections) (IHC-P). GTX83627

GTX83627 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83627 GLUT5 antibody [14C8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GLUT5 antibody [14C8] used in Western Blot (WB). GTX83627

GTX83627 WB Image

WB analysis of various cell lines using GTX83627 GLUT5 antibody [14C8].
Loading : 35 ug per lane
Dilution : 1:200

Anti-GLUT5 antibody [14C8] used in Western Blot (WB). GTX83627

GTX83627 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GLUT5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83627 GLUT5 antibody [14C8].
Loading : 5 ug per lane

Anti-GLUT5 antibody [14C8] used in Immunoprecipitation (IP). GTX83627

GTX83627 IP Image

IP analysis of DDDDK tagged GLUT5 overexpressed HEK293T lysate using GTX83627 GLUT5 antibody [14C8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519