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GLUT5 antibody [9F3]

Overexpression
Anti-GLUT5 antibody [9F3] used in Western Blot (WB). GTX83628
Anti-GLUT5 antibody [9F3] used in Western Blot (WB). GTX83628
Anti-GLUT5 antibody [9F3] used in Flow cytometry (FACS). GTX83628
Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628
Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628
Anti-GLUT5 antibody [9F3] used in Immunoprecipitation (IP). GTX83628
Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628
Anti-GLUT5 antibody [9F3] used in Flow cytometry (FACS). GTX83628

Cat. No. GTX83628

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9F3

Isotype

 IgG1

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($519)
See all GLUT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10728195

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 2 member 5 , GLUT-5 , GLUT5

Cellular Localization

Apical cell membrane,Cell membrane

Background

Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.

Database

Research Area

DATA IMAGES

Anti-GLUT5 antibody [9F3] used in Western Blot (WB). GTX83628

GTX83628 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GLUT5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83628 GLUT5 antibody [9F3].
Loading : 5 ug per lane

Anti-GLUT5 antibody [9F3] used in Western Blot (WB). GTX83628

GTX83628 WB Image

WB analysis of various cell lines using GTX83628 GLUT5 antibody [9F3].
Loading : 35 ug per lane

Anti-GLUT5 antibody [9F3] used in Flow cytometry (FACS). GTX83628

GTX83628 FACS Image

FACS analysis of HeLa cells using GTX83628 GLUT5 antibody [9F3].
Red : Primary antibody
Blue : Negative control antibody

Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628

GTX83628 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83628 GLUT5 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628

GTX83628 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83628 GLUT5 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GLUT5 antibody [9F3] used in Immunoprecipitation (IP). GTX83628

GTX83628 IP Image

IP analysis of DDDDK tagged GLUT5 overexpressed HEK293T lysate using GTX83628 GLUT5 antibody [9F3].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-GLUT5 antibody [9F3] used in IHC (Paraffin sections) (IHC-P). GTX83628

GTX83628 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83628 GLUT5 antibody [9F3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-GLUT5 antibody [9F3] used in Flow cytometry (FACS). GTX83628

GTX83628 FACS Image

FACS analysis of Jurkat cells using GTX83628 GLUT5 antibody [9F3].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

Application Reference

Evangelista-Silva PH et al. Life Sci 2021; 279 : 119672 Intestinal GLUT5 and FAT/CD36 transporters and blood glucose are reduced by a carotenoid/MUFA-rich oil in high-fat fed mice.
Application : WB
Reactivity : Mouse
Torelli Hijo AH et al. Heliyon 2019; 5 (10) : e02611 High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity.
Application : WB
Reactivity : Mouse
Andressa Harumi Torelli Hijo et al. Heliyon 2019; 5 (10) : e02611 High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity.
Application : WB
Reactivity : Mouse
Losacco MC et al. Life Sci 2018; (Epub) High-fat diet affects gut nutrients transporters in hypo and hyperthyroid mice by PPAR-a independent mechanism.
Hsieh CC et al. Oncotarget 2016; (Epub) Elevation of β-galactoside α2,6-sialyltransferase 1 in a fructoseresponsive manner promotes pancreatic cancer metastasis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519