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GNAO1 antibody

Anti-GNAO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114439
Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439
Anti-GNAO1 antibody used in Western Blot (WB). GTX114439
Anti-GNAO1 antibody used in Western Blot (WB). GTX114439
Anti-GNAO1 antibody used in Immunoprecipitation (IP). GTX114439
Anti-GNAO1 antibody used in Western Blot (WB). GTX114439
Anti-GNAO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114439
Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439
Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439
Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439
Anti-GNAO1 antibody used in Western Blot (WB). GTX114439

Cat. No. GTX114439

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GNAO1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

mouse brain , mouse lung , U87-MG , SK-N-SH , IMR32 , SK-N-AS , rat brain , rat lung

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAO1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620804

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha o1 , EIEE17 , G-ALPHA-o , GNAO , HLA-DQB1 , NEDIM

Database

Research Area

DATA IMAGES

Anti-GNAO1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX114439

GTX114439 IHC-Fr Image

GNAO1 antibody detects GNAO1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439

GTX114439 IHC-P Image

GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GNAO1 antibody (GTX114439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GNAO1 antibody used in Western Blot (WB). GTX114439

GTX114439 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAO1 antibody used in Western Blot (WB). GTX114439

GTX114439 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAO1 antibody used in Immunoprecipitation (IP). GTX114439

GTX114439 IP Image

Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody (GTX114439).
Western blot analysis was performed using GNAO1 antibody (GTX114439).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GNAO1 antibody used in Western Blot (WB). GTX114439

GTX114439 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAO1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114439

GTX114439 ICC/IF Image

GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439

GTX114439 IHC-P Image

GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439

GTX114439 IHC-P Image

GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAO1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114439

GTX114439 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNAO1 antibody (GTX114439) at 1:300 dilution.

Anti-GNAO1 antibody used in Western Blot (WB). GTX114439

GTX114439 WB Image

Sample (30 μg of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
GTX114439 diluted at 1:5000

REFERENCE

Application Reference

Dietschi Q et al. Sci Adv 2022; 8 (46) : eabn7450 Clustering of vomeronasal receptor genes is required for transcriptional stability but not for choice.
MonserratP?rez-Ram?rez et al. Clinical Neurology and Neurosurgery 2019; (Epub) Pediatric ependymoma: GNAO1, ASAH1, IMMT and IPO7 protein expression and 5-year prognosis correlation.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169