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GOT1 antibody

Anti-GOT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54155
Anti-GOT1 antibody used in Western Blot (WB). GTX54155
Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155
Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155
Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155

Cat. No. GTX54155

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all GOT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , cAspAT , cCAT

Cellular Localization

Cytoplasm

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GOT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54155

GTX54155 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54155 GOT1 antibody.
Blue : DAPI

Anti-GOT1 antibody used in Western Blot (WB). GTX54155

GTX54155 WB Image

WB analysis of various sample lysates using GTX54155 GOT1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155

GTX54155 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX54155 GOT1 antibody.
Dilution : 1:100

Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155

GTX54155 IHC-P Image

IHC-P analysis of human colon tissue using GTX54155 GOT1 antibody.
Dilution : 1:100

Anti-GOT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54155

GTX54155 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54155 GOT1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399