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GPAA1 antibody

KO/KD
Anti-GPAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115131
Anti-GPAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115131
Anti-GPAA1 antibody used in Western Blot (WB). GTX115131
Anti-GPAA1 antibody used in Western Blot (WB). GTX115131

Cat. No. GTX115131

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GPAA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPAA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycosylphosphatidylinositol anchor attachment 1 , GAA1 , GPIBD15 , hGAA1

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115131

GTX115131 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPAA1(GTX115131) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPAA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX115131

GTX115131 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GPAA1(GTX115131) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPAA1 antibody used in Western Blot (WB). GTX115131

GTX115131 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX115131 diluted at 1:1000

Anti-GPAA1 antibody used in Western Blot (WB). GTX115131

GTX115131 WB Image

KO/KD

The data was published in the 2022 in PLoS Pathog. PMID: 35231079

REFERENCE

Application Reference

Sherman EJ et al. PLoS Pathog 2022; 18 (3) : e1010377 Identification of cell type specific ACE2 modifiers by CRISPR screening.
Application : WB
Reactivity : Human
Kim P et al. Transl Psychiatry 2019; 9 (1) : 6 Abnormal ER quality control of neural GPI-anchored proteins via dysfunction in ER export processing in the frontal cortex of elderly subjects with schizophrenia.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169