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GPI antibody [1B7D7]

Anti-GPI antibody [1B7D7] used in IHC (Paraffin sections) (IHC-P). GTX83039
Anti-GPI antibody [1B7D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83039
Anti-GPI antibody [1B7D7] used in Western Blot (WB). GTX83039
Anti-GPI antibody [1B7D7] used in Western Blot (WB). GTX83039

Cat. No. GTX83039

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B7D7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Rat
Package
100 μl ($409)
See all GPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GPI expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm,Secreted

Background

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-GPI antibody [1B7D7] used in IHC (Paraffin sections) (IHC-P). GTX83039

GTX83039 IHC-P Image

IHC-P analysis of human Kidney tissue using GTX83039 GPI antibody [1B7D7].

Anti-GPI antibody [1B7D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83039

GTX83039 ICC/IF Image

ICC/IF analysis of L-02 cells using GTX83039 GPI antibody [1B7D7].
Green : GPI
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-GPI antibody [1B7D7] used in Western Blot (WB). GTX83039

GTX83039 WB Image

WB analysis of HepG2 (1) , SMMC-7721 (2) cell and rat liver tissue lysate (3) using GTX83039 GPI antibody [1B7D7].

Anti-GPI antibody [1B7D7] used in Western Blot (WB). GTX83039

GTX83039 WB Image

WB analysis of HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GPI cDNA (2) using GTX83039 GPI antibody [1B7D7].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409