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GPR43 antibody

Anti-GPR43 antibody used in Western Blot (WB). GTX00816
Anti-GPR43 antibody used in Western Blot (WB). GTX00816
Anti-GPR43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00816
Anti-GPR43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00816

Cat. No. GTX00816

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Mouse, Rat
Package
50 μl ($639)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

This antibody is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat intracellular, C-terminus FFAR2 (Accession Q76EI6).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cell membrane

Database

Research Area

DATA IMAGES

Anti-GPR43 antibody used in Western Blot (WB). GTX00816

GTX00816 WB Image

WB analysis of mouse colon tissue lysate using GTX00816 GPR43 antibody.
Lane 1 : Primary antibody
Lane 2 : Primary antibody preincubated with the negative control antigen
Dilution : 1:200

Anti-GPR43 antibody used in Western Blot (WB). GTX00816

GTX00816 WB Image

WB analysis of rat stomach (lanes 1 and 3) and small intestine (lanes 2 and 4) tissue lysates using GTX00816 GPR43 antibody.
Lane 1, 2 : Primary antibody
Lane 3, 4 : Primary antibody preincubated with the negative control antigen
Dilution : 1:500

Anti-GPR43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00816

GTX00816 IHC-Fr Image

IHC-Fr analysis of rat hippocampus using GTX00816 GPR43 antibody. Staining showed in rat hippocampus showed in astrocyte outlines (arrows) most intensely in the hilus (H). The staining result of antibody pre-incubate with blocking peptide showed in figure B.
Green : Primary antibody
Blue : DAPI
Dilution : 1:300

Anti-GPR43 antibody used in IHC (Frozen sections) (IHC-Fr). GTX00816

GTX00816 IHC-Fr Image

IHC-Fr analysis of mouse hippocampus using GTX00816 GPR43 antibody. Staining in the mouse hippocampus showed in the pyramidal layer (P) and in astrocyte outlines (arrows). The staining result of antibody pre-incubate with blocking peptide showed in figure B.
Green : Primary antibody
Blue : DAPI
Dilution : 1:300

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639