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GPX2 antibody [C1C3]

KO/KD Orthogonal
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100292
Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292
Anti-GPX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX100292
Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

Cat. No. GTX100292

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GPX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GPX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240895

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 2 , GI-GPx , GPRP , GPRP-2 , GPx-2 , GPx-GI , GSHPX-GI , GSHPx-2

Cellular Localization

Cytoplasm

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

GPX2 antibody detects GPX2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody (GTX100292) diluted by 1:500

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2(GTX100292) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPX2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100292

GTX100292 ICC/IF Image

GPX2 antibody [C1C3] detects GPX2 protein at granule by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GPX2 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX100292

GTX100292 IHC-P Image

GPX2 antibody [C1C3] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
GPX2 stained by GPX2 antibody [C1C3] (GTX100292) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

GPX2 antibody [C1C3] detects GPX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12 % SDS-PAGE, and blotted with GPX2 antibody [C1C3] (GTX100292) diluted by 1:1000

Anti-GPX2 antibody [C1C3] used in Immunoprecipitation (IP). GTX100292

GTX100292 IP Image

Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 μg of GPX2 antibody [C1C3] (GTX100292).
Western blot analysis was performed using GPX2 antibody [C1C3] (GTX100292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GPX2 antibody [C1C3] used in Western Blot (WB). GTX100292

GTX100292 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Brzozowa-Zasada M et al. Int J Mol Sci 2023; 24 (19) Immunohistochemical Expression of Glutathione Peroxidase-2 (Gpx-2) and Its Clinical Relevance in Colon Adenocarcinoma Patients.
Wen QL et al. Mol Cell Endocrinol 2021; 523 : 111144 Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: Involvement of oxidative stress.
Application : WB, IHC-P
Reactivity : Human
Jiaqian Xu et al. Oxid Med Cell Longev 2020; 2020 : 6240125 Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion.
Application : WB
Reactivity : Human
Moreno Leon L et al. Oncogene 2019; (Epub) The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress.
Application : WB
Reactivity : Human
Wu YL et al. Free Radic Biol Med 2018; 115 : 309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Brzozowa-Zasada M et al. Int J Mol Sci 2023; 24 (19) Immunohistochemical Expression of Glutathione Peroxidase-2 (Gpx-2) and Its Clinical Relevance in Colon Adenocarcinoma Patients.
Wen QL et al. Mol Cell Endocrinol 2021; 523:111144 Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: Involvement of oxidative stress.
Application : WB, IHC-P
Reactivity : Human
Jiaqian Xu et al. Oxid Med Cell Longev 2020; 2020:6240125 Mild Oxidative Stress Reduces NRF2 SUMOylation to Promote Kras/Lkb1/Keap1 Mutant Lung Adenocarcinoma Cell Migration and Invasion.
Application : WB
Reactivity : Human
Moreno Leon L et al. Oncogene 2019; (Epub) The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress.
Application : WB
Reactivity : Human
Wu YL et al. Free Radic Biol Med 2018; 115:309-317 Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Application : WB
Reactivity : Human
Saxena A et al. Cancer Biol Ther 2017;:0 Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Application : WB
Reactivity : Human
Kannan N et al. Proc Natl Acad Sci U S A 2014; 111 (21):7789-94 Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169