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GPX3 antibody, Internal

Anti-GPX3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89142
Anti-GPX3 antibody, Internal used in Western Blot (WB). GTX89142
Anti-GPX3 antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX89142
Anti-GPX3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89142

Cat. No. GTX89142

Reference 4

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Pig
Package
100 μg ($399)
See all GPX3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 20μg/ml
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89142-PEP).

Product Note

This antibody may cross-react with epididymis-specific GPX5 and olfactory epithelium-speciific GPX6

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NQFGKQEPGENSE, from the internal region of the protein sequence according to NP_002075.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10723471

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione peroxidase 3 , GPx-P , GSHPx-3 , GSHPx-P

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-GPX3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89142

GTX89142 IHC-P Image

IHC-P analysis of human colon using GTX89142 GPX3 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

Anti-GPX3 antibody, Internal used in Western Blot (WB). GTX89142

GTX89142 WB Image

WB analysis of mouse heart lysate using GTX89142 GPX3 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GPX3 antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX89142

GTX89142 IHC-Fr Image

IHC-Fr analysis of PFA-perfused pig kidney using GTX89142 GPX3 antibody, Internal.
Antigen retrieval : citrate buffer pH 3
Dilution : 20μg/ml

Anti-GPX3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89142

GTX89142 IHC-P Image

IHC-P analysis of human placenta using GTX89142 GPX3 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml

REFERENCE

Application Reference

David E Lee et al. JCSM Rapid Commun 2021; 4 (1) : 3月15日 Cancer-induced Cardiac Atrophy Adversely Affects Myocardial Redox State and Mitochondrial Oxidative Characteristics.
Application : WB
Reactivity : Mouse
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Application : WB
Reactivity : Mouse
Rosa-Caldwell ME et al. Int J Mol Sci 2020; 21 (21) Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia.
Brown JL et al. J Cachexia Sarcopenia Muscle 2017; (Epub) Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399