APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX22520-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GLFPANYVAPMTR, from the C Terminus of the protein sequence according to NP_004801.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GRB2 related adaptor protein 2 , GADS , GRAP-2 , GRB2L , GRBLG , GRID , GRPL , GrbX , Grf40 , Mona , P38
Cellular Localization
Nucleus , Cytoplasm , Endosome
Background
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
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GTX22520 WB Image
WB analysis of Jurkat lysate using GTX22520 GRAP2 antibody. Dilution : 0.3μg/ml Loading : 30μg protein in RIPA buffer
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GTX22520 WB Image
WB analysis of HEK293 overexpressing GRAP2 (mock transfection in first lane) using GTX22520 GRAP2 antibody.
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REFERENCE
There are currently no references for GRAP2 antibody (GTX22520). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GRAP2 antibody (GTX22520). Be the first to share your experience with this product.