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GRB2 antibody

Anti-GRB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32632
Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32632
Anti-GRB2 antibody used in Western Blot (WB). GTX32632

Cat. No. GTX32632

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GRB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRB2 (NP_002077.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

growth factor receptor bound protein 2 , ASH , EGFRBP-GRB2 , Grb3-3 , MST084 , MSTP084 , NCKAP2

Cellular Localization

Nucleus,Cytoplasm,Endosome,Golgi apparatus

Background

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GRB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32632

GTX32632 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32632 GRB2 antibody.
Blue : DAPI

Anti-GRB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX32632

GTX32632 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32632 GRB2 antibody.
Dilution : 1:100

Anti-GRB2 antibody used in Western Blot (WB). GTX32632

GTX32632 WB Image

WB analysis of various sample lysates using GTX32632 GRB2 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399