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GRK1 antibody [D11]

Anti-GRK1 antibody [D11] used in IHC (Frozen sections) (IHC-Fr). GTX22776
Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776
Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776
Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776

Cat. No. GTX22776

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 D11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, IP, IHC

Reactivity

 Human, Mouse, Bovine
Package
100 μg ($399)
See all GRK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:20-1:200
IHC-Fr 1:1,000
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

64 kDa. ( Note )

Product Note

This antibody does not cross-react with other G protein-coupled kinases. It recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GRK1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 1 , GPRK1 , RHOK , RK

Cellular Localization

Membrane

Background

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GRK1 antibody [D11] used in IHC (Frozen sections) (IHC-Fr). GTX22776

GTX22776 IHC-Fr Image

IHC-Fr analysis of bovine retina tissue using GTX22776 GRK1 antibody [D11].

Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776

GTX22776 ICC/IF Image

ICC/IF analysis of human cells using GTX22776 GRK1 antibody [D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:20 overnight at 4ºC

Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776

GTX22776 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX22776 GRK1 antibody [D11]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

Anti-GRK1 antibody [D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22776

GTX22776 ICC/IF Image

ICC/IF analysis of murine cells using GTX22776 GRK1 antibody [D11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:200 and incubated overnight in a humidified chamber

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399