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GRK2 antibody

KO/KD Overexpression
Anti-GRK2 antibody used in Western Blot (WB). GTX130012
Anti-GRK2 antibody used in Western Blot (WB). GTX130012
Anti-GRK2 antibody used in Immunoprecipitation (IP). GTX130012
Anti-GRK2 antibody used in Western Blot (WB). GTX130012
Anti-GRK2 antibody used in Western Blot (WB). GTX130012

Cat. No. GTX130012

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GRK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , ADRBK1-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886156

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled receptor kinase 2 , ADRBK1 , BARK1 , BETA-ARK1

Background

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

Untreated (–) and treated (+) Jurkat whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody used in Immunoprecipitation (IP). GTX130012

GTX130012 IP Image

Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody (GTX130012).
Western blot analysis was performed using GRK2 antibody (GTX130012).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

KO/KD

Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GRK2 antibody used in Western Blot (WB). GTX130012

GTX130012 WB Image

GRK2 antibody detects GRK2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody (GTX130012) diluted at a dilution of 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169