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GRP94 antibody [2H3]

Anti-GRP94 antibody [2H3] used in Western Blot (WB). GTX50064
Anti-GRP94 antibody [2H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50064

Cat. No. GTX50064

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H3

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($449)
See all GRP94 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Immunofluorescence: Use at 1:100 ~ 1:200.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human TRA1 (676-803aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GRP94 antibody [2H3] used in Western Blot (WB). GTX50064

GTX50064 WB Image

The cell lysates of hES(20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-GRP94 antibody [2H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50064

GTX50064 ICC/IF Image

Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449