GeneTex

  • Country / Location Selection

United States (US)

GRP94 (phospho Ser254) antibody

Anti-GRP94 (phospho Ser254) antibody used in Western Blot (WB). GTX79049

Cat. No. GTX79049

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all GRP94 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-Sp-D-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11173193

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ECGP,GP96,GRP94,HEL35,HELS125m,HSP90B1,TRA1,heat shock protein 90 beta family member 1,gp96,ERp99,Endoplasmin

Cellular Localization

Endoplasmic reticulum lumen,Melanosome

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GRP94 (phospho Ser254) antibody used in Western Blot (WB). GTX79049

GTX79049 WB Image

WB analysis of HT29 cells treated with anisomycin (500ng/ml, 30min) using GTX79049 GRP94 (phospho Ser254) antibody.

REFERENCE

There are currently no references for GRP94 (phospho Ser254) antibody (GTX79049). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GRP94 (phospho Ser254) antibody (GTX79049). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399