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GSS antibody [AT12F2]

Anti-GSS antibody [AT12F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57671
Anti-GSS antibody [AT12F2] used in Western Blot (WB). GTX57671

Cat. No. GTX57671

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT12F2

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all GSS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT12F2 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GSS protein

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione synthetase , GSHS , GSS , HELS64p , HELS88n , Glutathione Synthetase

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GSS antibody [AT12F2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57671

GTX57671 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57671 GSS antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-GSS antibody [AT12F2] used in Western Blot (WB). GTX57671

GTX57671 WB Image

WB analysis of various samples using GTX57671 GSS antibody.
Lane 1 : MDA-MB231 whole cell lysate
Lane 2 : WiDr whole cell lysate
Lane 3 : SW480 whole cell lysate
Lane 4 : A427 whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Lane 6 : 293T whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : HT1080 whole cell lysate
Lane 9 : U87-MG whole cell lysate
Lane 10 : A549 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399