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GST tag antibody

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736
Anti-GST tag antibody used in Western Blot (WB). GTX110736
Anti-GST tag antibody used in Western Blot (WB). GTX110736
Anti-GST tag antibody used in Western Blot (WB). GTX110736

Cat. No. GTX110736

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all GST tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to GST

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949427

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GST

Research Area

DATA IMAGES

Anti-GST tag antibody used in Immunoprecipitation (IP). GTX110736

GTX110736 IP Image

GST-tagged protein (Mw: 45 kDa) was immunoprecipitated from E. coli lysate using 8 ug of anti-GST antibody (GTX110736).
The precipitated protein was detected by GTX110736 diluted at 1:3000.

Anti-GST tag antibody used in Western Blot (WB). GTX110736

GTX110736 WB Image

IPTG-induced cell lysate from E. coli expressing GST-tag tagged proteins were separated by 10% SDS-PAGE, and the membrane was blotted with GST tag antibody (GTX110736) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GST tag antibody used in Western Blot (WB). GTX110736

GTX110736 WB Image

The data was published in the journal BMC Biol in 2017.PMID: 29183317

Anti-GST tag antibody used in Western Blot (WB). GTX110736

GTX110736 WB Image

The data was published in the journal Elife in 2018.PMID: 30516470

REFERENCE

Application Reference

Hsieh YL et al. Cell Rep 2019; 29 (8) : 2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Application : WB
Lai TC et al. Sci Rep 2019; 9 (1) : 5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Chen YC et al. Elife 2018; 7 Glucose intake hampers PKA-regulated HSP90 chaperone activity.
Matsuyama T et al. Vaccine 2018; (Epub) Antibody profiling using a recombinant protein-based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model.
Application : ELISA
Hsu WL et al. BMC Biol 2017; 15 (1) : 112 Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2.
Application : WB
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REVIEW

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Application Reference
Hsieh YL et al. Cell Rep 2019; 29 (8):2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Application : WB
Lai TC et al. Sci Rep 2019; 9 (1):5304 Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
Application : WB
Chen YC et al. Elife 2018; 7 Glucose intake hampers PKA-regulated HSP90 chaperone activity.
Matsuyama T et al. Vaccine 2018; (Epub) Antibody profiling using a recombinant protein-based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model.
Application : ELISA
Hsu WL et al. BMC Biol 2017; 15 (1):112 Smad4 SUMOylation is essential for memory formation through upregulation of the skeletal myopathy gene TPM2.
Application : WB
Shih JW et al. Nat Commun 2017; 8:15874 Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression.
Application : WB
Tai DJ et al. Nat Commun 2016; 7:10552 MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome.
Application : WB
Chen HA et al. Hepatology 2016; angptl1 antagonizes met receptor activity to repress * resistance and cancer stemness in hepatocellular carcinoma cells.
Application : WB
Busch DJ et al. Mol Biol Cell 2014; Acute increase of α-synuclein inhibits synaptic vesicle recycling evoked during intense stimulation.
Application : WB
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169