APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse liver , HepG2
Product Note
Based on sequence homology, this antibody may cross react with other GSTA family members.
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
6 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein corresponding to human GSTA1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_1950465
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutathione S-transferase alpha 1 , GST-epsilon , GST2 , GSTA1-1 , GTH1
Cellular Localization
Cytoplasm
Background
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX108012 WB Image
GSTA1 antibody detects GSTA1 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
12 % SDS-PAGE
GSTA1 antibody (GTX108012) dilution: 1:5000
|
|
|
GTX108012 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GSTA1(GTX108012) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX108012 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
12% SDS PAGE
GSTA1 antibody
GTX108012 diluted at 1:1000
|
|
|
GTX108012 ICC/IF Image
Immunofluorescence analysis of methanol-fixed Hep3B, using GSTA1(GTX108012) antibody at 1:500 dilution.
|
REFERENCE
There are currently no references for GSTA1 antibody (GTX108012). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GSTA1 antibody (GTX108012). Be the first to share your experience with this product.
