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GSTT1 antibody

KO/KD
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in Immunoprecipitation (IP). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250
Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

Cat. No. GTX109250

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GSTT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

rat liver

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human GSTT1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950469

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase theta 1

Cellular Localization

Cytoplasm

Background

Glutathione S-transferase (GST) theta 1 (GSTT1) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. The GSTT1 and GSTT2 share 55% amino acid sequence identity and both of them were claimed to have an important role in human carcinogenesis. The GSTT1 gene is located approximately 50kb away from the GSTT2 gene. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by western blot analysis.
A. 50 μg rat liver lysate/extract
12% SDS-PAGE
GSTT1 antibody (GTX109250) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GSTT1 antibody used in Immunoprecipitation (IP). GTX109250

GTX109250 IP Image

Immunoprecipitation of GSTT1 protein from HepG2 whole cell extracts using 5 μg of GSTT1 antibody (GTX109250).
Western blot analysis was performed using GSTT1 antibody (GTX109250).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GSTT1 antibody (GTX109250) diluted by 1:1000.

Anti-GSTT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109250

GTX109250 IHC-P Image

GSTT1 antibody detects GSTT1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatoma.
GSTT1 antibody (GTX109250) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

GSTT1 antibody detects GSTT1 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with GSTT1 antibody (GTX109250) diluted by 1:1000.

Anti-GSTT1 antibody used in Western Blot (WB). GTX109250

GTX109250 WB Image

KO/KD

The data was published in the journal Nat Commun in 2018. PMID: 30446643

REFERENCE

Application Reference

Zhou T et al. Nat Commun 2018; 9 (1) : 4815 A hPSC-based platform to discover gene-environment interactions that impact human β-cell and * neuron survival.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169