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GSTT2 antibody [8A10]

Overexpression
Anti-GSTT2 antibody [8A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84398
Anti-GSTT2 antibody [8A10] used in Western Blot (WB). GTX84398
Anti-GSTT2 antibody [8A10] used in Flow cytometry (FACS). GTX84398
Anti-GSTT2 antibody [8A10] used in Flow cytometry (FACS). GTX84398
Anti-GSTT2 antibody [8A10] used in Western Blot (WB). GTX84398

Cat. No. GTX84398

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($509)
See all GSTT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GSTT2 (NP_000845) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10730094

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutathione S-transferase theta 2 (gene/pseudogene)

Cellular Localization

Cytoplasm

Background

Glutathione S-transferase (GSTs) theta 2 (GSTT2) is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: Alpha, Mu, Pi, Theta, and Zeta. The theta class members GSTT1 and GSTT2 share 55% amino acid sequence identity and both are thought to have an important role in human carcinogenesis. The theta genes have a similar structure, being composed of five exons with identical exon/intron boundaries. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GSTT2 antibody [8A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84398

GTX84398 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GSTT2 plasmid using GTX84398 GSTT2 antibody [8A10].

Anti-GSTT2 antibody [8A10] used in Western Blot (WB). GTX84398

GTX84398 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GSTT2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84398 GSTT2 antibody [8A10].
Loading : 5 ug per lane

Anti-GSTT2 antibody [8A10] used in Flow cytometry (FACS). GTX84398

GTX84398 FACS Image

FACS analysis of HEK293T cells transfected with either GSTT2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84398 GSTT2 antibody [8A10].

Anti-GSTT2 antibody [8A10] used in Flow cytometry (FACS). GTX84398

GTX84398 FACS Image

FACS analysis of HeLa cells using GTX84398 GSTT2 antibody [8A10].
Red : Primary antibody
Blue : Negative control antibody

Anti-GSTT2 antibody [8A10] used in Western Blot (WB). GTX84398

GTX84398 WB Image

WB analysis of various cell lines using GTX84398 GSTT2 antibody [8A10].
Loading : 35 ug per lane

REFERENCE

Application Reference

Wang C et al. Mol Nutr Food Res 2018; (Epub) Histone Methyltransferase Setd7 Regulates Nrf2 Signaling Pathway by Phenethyl Isothiocyanate and Ursolic Acid in Human Prostate Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 509