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GTF2H1 antibody [N1C1]

Comparison
Anti-GTF2H1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111804
Anti-GTF2H1 antibody [N1C1] used in Western Blot (WB). GTX111804
Anti-GTF2H1 antibody [N1C1] used in Western Blot (WB). GTX111804

Cat. No. GTX111804

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GTF2H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HepG2 , A549 , H1299 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GTF2H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175381

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

general transcription factor IIH subunit 1 , BTF2 , P62 , TFB1 , TFIIH

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-GTF2H1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111804

GTX111804 ICC/IF Image

GTF2H1 antibody [N1C1] detects GTF2H1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GTF2H1 stained by GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-GTF2H1 antibody [N1C1] used in Western Blot (WB). GTX111804

GTX111804 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with GTF2H1 antibody [N1C1] (GTX111804) diluted at 1:1000 and competitor's antibody (SC-292) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-GTF2H1 antibody [N1C1] used in Western Blot (WB). GTX111804

GTX111804 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX111804 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169