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Galanin antibody, Internal

Anti-Galanin antibody, Internal used in Western Blot (WB). GTX88303
Anti-Galanin antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX88303
Anti-Galanin antibody, Internal used in Western Blot (WB). GTX88303

Cat. No. GTX88303

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P Assay dependent
IHC-Fr 0.2-0.5μg/ml

Note :

IHC-Fr
Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus.

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88303-PEP).

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HRSFSDKNGLTSK, from the internal region of the protein sequence according to NP_057057.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

galanin and GMAP prepropeptide , ETL8 , GAL-GMAP , GALN , GLNN , GMAP

Cellular Localization

Secreted

Background

This gene encodes a neuroendocrine peptide that is widely expressed in the central and peripheral nervous systems and also the gastrointestinal tract, pancreas, adrenal gland and urogenital tract. The encoded protein is a precursor that is proteolytically processed to generate two mature peptides: galanin and galanin message-associated peptide (GMAP). Galanin has diverse physiological functions including nociception, feeding and energy homeostasis, osmotic regulation and water balance. GMAP has been demonstrated to possess antifungal activity and hypothesized to be part of the innate immune system. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-Galanin antibody, Internal used in Western Blot (WB). GTX88303

GTX88303 WB Image

WB analysis of mouse (A) and rat (B) brain lysate using GTX88303 Galanin antibody, Internal.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Galanin antibody, Internal used in IHC (Frozen sections) (IHC-Fr). GTX88303

GTX88303 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamus using GTX88303 Galanin antibody, Internal.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.3μg/ml

Anti-Galanin antibody, Internal used in Western Blot (WB). GTX88303

GTX88303 WB Image

WB analysis of human cerebellum lysate using GTX88303 Galanin antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399