APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 5 μg/ml |
| 1:50-1:500 |
| 5 μg/ml |
| 1-2 μg/test |
| Assay dependent |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Using enzymatic digestion analysis, this antibody reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Partially purified rat GR.
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
nuclear receptor subfamily 3, group C, member 1 , GR , Gcr , Grl
Cellular Localization
Nucleus,Mitochondrion
Background
glucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]
Database
Research Area
DATA IMAGES
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/templates/default/js/lightbox/images/loading.gif "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
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GTX22768 FACS Image
FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 2 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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GTX22768 WB Image
WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].
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GTX22768 ICC/IF Image
ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX22768 FACS Image
FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
|
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GTX22768 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
|
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GTX22768 ICC/IF Image
ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX22768 FACS Image
FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue).
Dilution : 1 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde
Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
|
REFERENCE
There are currently no references for Glucocorticoid Receptor antibody [BuGR2] (GTX22768). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Glucocorticoid Receptor antibody [BuGR2] (GTX22768). Be the first to share your experience with this product.
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_134_FACS_w_23060620_524.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Western Blot (WB). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_1586_WB_w_23060620_941.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Western Blot (WB). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_410_ICC-IF_w_23060620_543.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_133_FACS_w_23060620_224.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_411_ICC-IF_w_23060620_802.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_412_ICC-IF_w_23060620_451.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/small/GTX22768/GTX22768_132_FACS_w_23060620_102.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_134_FACS_w_23060620_524.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Western Blot (WB). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_1586_WB_w_23060620_941.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Western Blot (WB). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_410_ICC-IF_w_23060620_543.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_133_FACS_w_23060620_224.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_411_ICC-IF_w_23060620_802.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_412_ICC-IF_w_23060620_451.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22768")
![Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768](/upload/website/prouct_img/normal/GTX22768/GTX22768_132_FACS_w_23060620_102.webp "Anti-Glucocorticoid Receptor antibody [BuGR2] used in Flow cytometry (FACS). GTX22768")