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Glycophorin C antibody

Anti-Glycophorin C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54084
Anti-Glycophorin C antibody used in Western Blot (WB). GTX54084

Cat. No. GTX54084

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Glycophorin C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human GYPC (NP_002092.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycophorin C (Gerbich blood group) , CD236 , CD236R , GE , GE:GPC:GPD:GYPD , GPC , GPD , GYPD , PAS-2 , PAS-2'

Background

Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-Glycophorin C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54084

GTX54084 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX54084 Glycophorin C antibody.
Blue : DAPI

Anti-Glycophorin C antibody used in Western Blot (WB). GTX54084

GTX54084 WB Image

WB analysis of various sample lysates using GTX54084 Glycophorin C antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399