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Grp75 antibody [9F8]

Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in Flow cytometry (FACS). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84331
Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in Flow cytometry (FACS). GTX84331
Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331
Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331
Anti-Grp75 antibody [9F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84331

Cat. No. GTX84331

Host

Mouse

Clonality

Monoclonal

Clone Name

9F8

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:1000
ICC/IF 1:50
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 9 , CRP40 , CSA , EVPLS , GRP-75 , GRP75 , HEL-S-124m , HSPA9B , MOT , MOT2 , MTHSP75 , PBP74 , SAAN , SIDBA4

Cellular Localization

Mitochondrion,Nucleolus

Background

Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.

Database

Research Area

DATA IMAGES

Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331

GTX84331 WB Image

WB analysis of various samples using GTX84331 Grp75 antibody [9F8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in Flow cytometry (FACS). GTX84331

GTX84331 FACS Image

FACS analysis of Jurkat cells using GTX84331 Grp75 antibody [9F8].
Red : Primary antibody
Blue : Negative control antibody

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84331

GTX84331 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Grp75 plasmid using GTX84331 Grp75 antibody [9F8].

Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331

GTX84331 WB Image

WB analysis of various cell lines using GTX84331 Grp75 antibody [9F8].
Loading : 35 ug per lane

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in Flow cytometry (FACS). GTX84331

GTX84331 FACS Image

FACS analysis of HeLa cells using GTX84331 Grp75 antibody [9F8].
Red : Primary antibody
Blue : Negative control antibody

Anti-Grp75 antibody [9F8] used in Western Blot (WB). GTX84331

GTX84331 WB Image

WB analysis of HEK293T cells transfected with Grp75 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84331 Grp75 antibody [9F8].
Loading : 5 ug per lane

Anti-Grp75 antibody [9F8] used in IHC (Paraffin sections) (IHC-P). GTX84331

GTX84331 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84331 Grp75 antibody [9F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-Grp75 antibody [9F8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84331

GTX84331 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84331 Grp75 antibody [9F8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519