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Grp75 antibody

KO/KD Orthogonal
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407
Anti-Grp75 antibody used in Immunoprecipitation (IP). GTX104407
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407
Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407
Anti-Grp75 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104407
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Anti-Grp75 antibody used in Western Blot (WB). GTX104407

Cat. No. GTX104407

Reference 4
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($399),
25 μl ($169)
See all Grp75 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

SCC-15

Product Note

Knockdown/Knockout validation was supported by customer review data.

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp75. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950527

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 9 , CRP40 , CSA , EVPLS , GRP-75 , GRP75 , HEL-S-124m , HSPA9B , MOT , MOT2 , MTHSP75 , PBP74 , SAAN , SIDBA4

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407

GTX104407 IHC-P Image

Grp75 antibody detects Grp75 protein at mitochondria in rat duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407

GTX104407 IHC-P Image

Grp75 antibody detects Grp75 protein at mitochondria in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407

GTX104407 IHC-P Image

Grp75 antibody detects Grp75 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in Immunoprecipitation (IP). GTX104407

GTX104407 IP Image

Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 μg of GRP75 antibody (GTX104407).
Western blot analysis was performed using GRP75 antibody (GTX104407) diluted at 1:2500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Grp75 antibody used in Western Blot (WB). GTX104407

GTX104407 WB Image

Whole cell extract (6 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody (GTX104407) diluted at 1:500.

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407

GTX104407 IHC-P Image

Grp75 antibody detects Grp75 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in IHC (Paraffin sections) (IHC-P). GTX104407

GTX104407 IHC-P Image

Grp75 antibody detects Grp75 protein at mitochondria in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Grp75 antibody (GTX104407) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Grp75 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104407

GTX104407 ICC/IF Image

Grp75 antibody detects Grp75 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Grp75 stained by Grp75 antibody (GTX104407) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Grp75 antibody used in Western Blot (WB). GTX104407

GTX104407 WB Image

Orthogonal

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX104407) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp75 antibody used in Western Blot (WB). GTX104407

GTX104407 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody (GTX104407) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Grp75 antibody used in Western Blot (WB). GTX104407

GTX104407 WB Image

Orthogonal

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

REFERENCE

Application Reference

Su YS et al. Front Microbiol 2020; 11 : 1656 The Heat Shock Protein 70 Family of Chaperones Regulates All Phases of the Enterovirus A71 Life Cycle.
Application : WB
Reactivity : Human
Bassoy EY et al. EMBO J 2017; 36 (11) : 1493-1512 ER-mitochondria contacts control surface glycan expression and sensitivity to killer lymphocytes in glioma stem-like cells.
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4) : 744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Chou HC et al. Proteome Sci 2014; 12 (1) : 2 Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Grp75 antibody Cat. No. GTX104407

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : SAS cells
Amount used : 20μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Date : Anonymous submitted on 10-Dec-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : kindey tissue
Amount used : 20μg
Blocking : 3% BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Grp75 antibody used in Western Blot (WB). GTX104407
Date : Anonymous submitted on 15-Jun-2015
Score :
Application Tested : WB
Sample Species : Pig
Sample : IPEC-J2 cells
Amount used : 5μg
Blocking : 5% skim milk, 22°C, 1Hr
Primary Antibody : 1:1000, 4°C, 14Hr
Figure Note : IPEC-J2 mitochondrial extract.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169