APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , HepG2
Predict Reactivity
Zebrafish, Bovine, Japanese Medaka, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.13 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11173017
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 5 , BIP , GRP78 , HEL-S-89n , MIF2
Cellular Localization
Endoplasmic reticulum lumen , Melanosome
Background
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX113340 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Grp78 antibody (GTX113340) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 WB Image
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX113340 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113340 ICC/IF Image
Grp78 antibody detects Grp78 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Grp78 antibody (GTX113340) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody (GTX113340) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX113340 IHC-P Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Grp78 stained by Grp78 antibody (GTX113340) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113340 WB Image
Grp78 antibody detects Grp78 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
7.5% SDS-PAGE
Grp78 antibody (GTX113340) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113340 ICC/IF Image
Grp78 antibody detects Grp78 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Grp78 protein stained by Grp78 antibody (GTX113340) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX113340 WB Image
The data was published in the journal J Biomed Sci in 2013. PMID: 24359494
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GTX113340 WB Image
The data was published in the journal PLoS One in 2017. PMID: 28234964
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GTX113340 WB Image
The data was published in the journal Biomed Res Int in 2020. PMID: 32071920
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REFERENCE
REVIEW
Grp78 antibody
Cat. No. GTX113340
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Rating
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( Average 5 based on 3 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 3 users reviews)
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Date :
Anonymous submitted on 15-Nov-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
CAL-27 shLu treated with Drug
Amount used :
30ug
Blocking :
5%milk/TBST, RT°C, 1Hr
Primary Antibody :
1:1000, 4°C, 18Hr
Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 14-May-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
3 μL
Blocking :
3% BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:10000, 4°C, 12Hr
