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H6PD antibody [2A7]

Overexpression
Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395
Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395
Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395
Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395
Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395
Anti-H6PD antibody [2A7] used in Flow cytometry (FACS). GTX84395
Anti-H6PD antibody [2A7] used in Flow cytometry (FACS). GTX84395
Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395
Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395
Anti-H6PD antibody [2A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84395

Cat. No. GTX84395

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2A7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all H6PD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase , CORTRD1 , G6PDH , GDH

Background

Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.

Database

Research Area

DATA IMAGES

Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395

GTX84395 WB Image

WB analysis of various cell lines using GTX84395 H6PD antibody [2A7].
Loading : 35 ug per lane

Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395

GTX84395 IHC-P Image

IHC-P analysis of human liver tissue using GTX84395 H6PD antibody [2A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395

GTX84395 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84395 H6PD antibody [2A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395

GTX84395 WB Image

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84395 H6PD antibody [2A7].
Loading : 10 ug per lane
Dilution : 1:500

Anti-H6PD antibody [2A7] used in Western Blot (WB). GTX84395

GTX84395 WB Image

Overexpression

WB analysis of HEK293T cells transfected with H6PD plasmid (Right) or empty vector (Left) for 48 hrs using GTX84395 H6PD antibody [2A7].
Loading : 5 ug per lane

Anti-H6PD antibody [2A7] used in Flow cytometry (FACS). GTX84395

GTX84395 FACS Image

FACS analysis of Jurkat cells using GTX84395 H6PD antibody [2A7].
Red : Primary antibody
Blue : Negative control antibody

Anti-H6PD antibody [2A7] used in Flow cytometry (FACS). GTX84395

GTX84395 FACS Image

FACS analysis of HEK293T cells transfected with either H6PD plasmid(Red) or empty vector control plasmid(Blue) using GTX84395 H6PD antibody [2A7].

Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395

GTX84395 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84395 H6PD antibody [2A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-H6PD antibody [2A7] used in IHC (Paraffin sections) (IHC-P). GTX84395

GTX84395 IHC-P Image

IHC-P analysis of human colon tissue using GTX84395 H6PD antibody [2A7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-H6PD antibody [2A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84395

GTX84395 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with H6PD plasmid using GTX84395 H6PD antibody [2A7].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519