HA tag antibody [GT423]

Name: HA tag antibody [GT423]
Brand: GeneTex
SKU: GTX628489
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-HA tag antibody [GT423] used in Western Blot (WB). GTX628489

Anti-HA tag antibody [GT423] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628489

Anti-HA tag antibody [GT423] used in Immunoprecipitation (IP). GTX628489

Anti-HA tag antibody [GT423] used in Western Blot and Immunoprecipitation (WB IP). GTX628489

Cat. No. GTX628489

Reference 12

Host	Mouse
Clonality	Monoclonal
Clone Name	GT423
Isotype	IgG2b
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all HA tag products

View product citations for antibody GTX628489 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:2000
IP	1:100-1:500

Not tested in other applications.

Positive Control

HA-tag fusion protein-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to HA tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888042

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HA tag antibody , HA antibody , HA epitope tag antibody

Research Area

DATA IMAGES

GTX628489 WB Image

HA tag antibody [GT423] detects HA tag protein by western blot analysis. HA tag-transfected and GST tag-transfected (30 μg) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628489 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with HA tag antibody [GT423] (GTX628489) diluted at 1:5000 and competitor's antibody (sc-7392) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX628489 ICC/IF Image

HA tag antibody [GT423] detects HA protein by immunofluorescent analysis.
Sample: HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HA stained by HA tag antibody [GT423] (GTX628489) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX628489 IP Image

HA tag antibody [GT423] immunoprecipitates HA-HIF-1α protein in IP experiments.
IP Sample: 400 μg cell lysate/extract of HA-HIF-1α transfected 293T cells
A. Control with 2 μg of preimmune mouse IgG
B. Immunoprecipitation of HA-fused protein by 2 μg of HA tag antibody [GT423] (GTX628489)
5% SDS-PAGE
The immunoprecipitated HA-HIF1α protein was detected by HIF-1α antibody (GTX127309) diluted at 1:1000.