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HADH antibody

KO/KD
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167
Anti-HADH antibody used in Western Blot (WB). GTX105167
Anti-HADH antibody used in Immunoprecipitation (IP). GTX105167

Cat. No. GTX105167

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all HADH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

GL261 , C8D30 , PC-12 , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HADH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxyacyl-CoA dehydrogenase , HAD , HADH1 , HADHSC , HCDH , HHF4 , MSCHAD , SCHAD

Cellular Localization

Mitochondrion matrix

Background

This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HADH antibody (GTX105167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 ug C8D30 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105167

GTX105167 ICC/IF Image

HADH antibody detects HADH protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: HADH protein stained by HADH antibody (GTX105167) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in IHC (Paraffin sections) (IHC-P). GTX105167

GTX105167 IHC-P Image

HADH antibody detects HADH protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
HADH antibody (GTX105167) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HADH antibody used in Western Blot (WB). GTX105167

GTX105167 WB Image

HADH antibody detects HADH protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
10 % SDS-PAGE
HADH antibody (GTX105167) dilution: 1:1000

Anti-HADH antibody used in Immunoprecipitation (IP). GTX105167

GTX105167 IP Image

Immunoprecipitation of HADH protein from HepG2 whole cell extracts using 5 μg of HADH antibody (GTX105167).
Western blot analysis was performed using HADH antibody (GTX105167).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Suzuki K et al. J Biol Chem 2023; 299 (10) : 105210 CLPX regulates mitochondrial fatty acid β-oxidation in liver?cells.
St-Louis JL et al. J Biol Chem 2023; 299 (8) : 104986 Deficiency of the metabolic enzyme SCHAD in pancreatic β-cells promotes amino acid-sensitive hypoglycemia.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169