APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89202-PEP).
Product Note
This antibody is expected to recognize all reported isoforms (NP_003940.1, NP_817084.1)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RYDFRYSEDREQ, from the internal region of the protein sequence according to NP_003940.2; NP_817084.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
huntingtin associated protein 1 , HAP2 , HIP5 , HLP , hHLP1
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton , Lysosome , Endoplasmic reticulum , Mitochondrion , Nucleus
Background
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX89202 WB Image
WB analysis of human brain (hippocampus) lysate using GTX89202 HAP1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89202 IHC-P Image
IHC-P analysis of human cortex using GTX89202 HAP1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for HAP1 antibody, Internal (GTX89202). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for HAP1 antibody, Internal (GTX89202). Be the first to share your experience with this product.
