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HAT1 antibody

Anti-HAT1 antibody used in Immunoprecipitation (IP). GTX110643
Anti-HAT1 antibody used in Western Blot (WB). GTX110643
Anti-HAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110643
Anti-HAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110643

Cat. No. GTX110643

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human HAT1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722082

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone acetyltransferase 1 , KAT1

Cellular Localization

Nucleus , Cytoplasm

Background

Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HAT1 antibody used in Immunoprecipitation (IP). GTX110643

GTX110643 IP Image

HAT1 antibody immunoprecipitates HAT1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HAT1 protein by 2 μg of HAT1 antibody (GTX110643) 12% SDS-PAGE The immunoprecipitated HAT1 protein was detected by HAT1 antibody (GTX110643) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-HAT1 antibody used in Western Blot (WB). GTX110643

GTX110643 WB Image

HAT1 antibody detects HAT1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HAT1 antibody (GTX110643) diluted by 1:10000.

Anti-HAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110643

GTX110643 ICC/IF Image

HAT1 antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HAT1 protein stained by HAT1 antibody [N1C3-2] (GTX110643) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-HAT1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110643

GTX110643 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon, using HAT1(GTX110643) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Zhang Q et al. Medicine (Baltimore) 2021; 100 (51) : e27868 Epigenetic modifications of tumor necrosis factor-alpha in joint cartilage tissue from osteoarthritis patients - CONSORT.
Application : ChIP assay
Reactivity : Human
Chiao-Yun Lin et al. J Mol Med (Berl). 2020; 98 (8) : 1175-1188 Synergistic effects of pazopanib and hyperthermia against uterine leiomyosarcoma growth mediated by downregulation of histone acetyltransferase 1.
Application : IHC-P
Reactivity : Human
Rouble A.N. et al. Journal of Thermal Biology 2018; 74 (Epub) : 71-76 Roles for lysine acetyltransferases during mammalian hibernation.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169