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HCN2 antibody

Anti-HCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82890

Cat. No. GTX82890

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all HCN2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 2 μg/ml
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Product Note

This antibody detects bands at ~100 kDa and ~115 kDa representing the non-glycosylated and glycosylated form of HCN2 in rat brain extract.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide: G(874) L D P Q D S A R S R L S S N L(889)

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2

Cellular Localization

Cell membrane

Background

may play a role in generation of neuronal pacemaker activity [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-HCN2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82890

GTX82890 ICC/IF Image

ICC/IF analysis of Neuro-2A cells using GTX82890 HCN2 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399