HDAC1 antibody

Cat. No. GTX20012

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP, ELISA, ChIP assay
Reactivity	Human, Mouse
Package	50 μg ($679)

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Datasheet
Document

View product citations for antibody GTX20012 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	1:200
IP	Assay dependent
ELISA	1:100 - 1:300
ChIP assay	2.4 μg

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

buffer

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

raised in rabbits against the C-terminal region of human HDAC1, using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone deacetylase 1 , GON-10 , HD1 , KDAC1 , RPD3 , RPD3L1

Cellular Localization

Nucleus

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 3065 HDAC1
UniProt: Q13547 HDAC1

Research Area

DATA IMAGES

GTX20012 ELISA Image

To determine the titer, an ELISA was performed using anti-HDAC1 crude serum, purified anti-HDAC1 antibodies (GTX20012), and the column flow through obtained from the antibody purification step. The antigen used was the C-terminal peptide used for immunization. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,250.

GTX20012 IP Image

Immunoprecipitation of HDAC1 was performed from HA-tagged HDAC1 transiently transfected HEK293T cell lysates (Panel A) or HeLa extract (Panel B) using GTX20012 HDAC1 antibody. WB was performed using anti- HA antibody or GTX20012 HDAC1 antibody as indicated.
Lane 1 and 4 : Beads only
Lane 2 and 5 : IP with 2 μg GTX20012 HDAC1 antibody
Lane 3 : IP with HA antibody as a confrol

GTX20012 ChIP assay Image

ChIP analysis of U-2OS cells using GTX20012 HDAC1 antibody.
Left : ChIP results using the anti-HDAC1 antibody (bar 2) or beads only (bar 1) and PCR primers specific for p21
Right : ChIP results using the anti-HDAC1 antibody (bar 4) or beads only (bar 3) and PCR primers for GAPDH (used as negative control).
ChIP reaction : 2.4μg antibody / 1 million cells

GTX20012 ICC/IF Image

ICC/IF analysis of mouse differentiated ES cells using GTX20012 HDAC1 antibody. Subsequently, RNA FISH (fluorescence in situ hybridization) was performed to detect Xist RNA (green signal). Nuclei were DAPI-stained to specifically label the DNA.
Fixation : formaldehyde
Permeabilization : Triton X-100
Dilution : 1:200 and incubated for 45 minutes at room temperature

REFERENCE

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SDS
Sodium Azide.pdf

Package

List Price ($)

50 μg

$ 679

Qty