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HDGF antibody

Anti-HDGF antibody used in Western Blot (WB). GTX54144
Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144
Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144
Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144
Anti-HDGF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54144
Anti-HDGF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54144

Cat. No. GTX54144

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HDGF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heparin binding growth factor , HMG1L2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-HDGF antibody used in Western Blot (WB). GTX54144

GTX54144 WB Image

WB analysis of various sample lysates using GTX54144 HDGF antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144

GTX54144 IHC-P Image

IHC-P analysis of rat liver tissue using GTX54144 HDGF antibody.
Dilution : 1:100

Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144

GTX54144 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX54144 HDGF antibody.
Dilution : 1:100

Anti-HDGF antibody used in IHC (Paraffin sections) (IHC-P). GTX54144

GTX54144 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX54144 HDGF antibody.
Dilution : 1:100

Anti-HDGF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54144

GTX54144 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54144 HDGF antibody.
Blue : DAPI
Dilution : 1:100

Anti-HDGF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54144

GTX54144 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54144 HDGF antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399