HES1 antibody [N1], N-term

Name: HES1 antibody [N1], N-term
Brand: GeneTex
SKU: GTX108356
Price: 399.00 USD

Anti-HES1 antibody [N1], N-term used in Western Blot (WB). GTX108356

Anti-HES1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108356

Anti-HES1 antibody [N1], N-term used in Immunoprecipitation (IP). GTX108356

Anti-HES1 antibody [N1], N-term used in Western Blot and Immunoprecipitation (WB IP). GTX108356

Cat. No. GTX108356

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all HES1 products

View product citations for antibody GTX108356 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:2000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

PC-12, Molt-4

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HES1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950487

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hes family bHLH transcription factor 1 , HES-1 , HHL , HRY , bHLHb39

Cellular Localization

Nucleus

Background

This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]

Database

Gene ID: 3280 HES1
UniProt: Q14469 HES1

Research Area

DATA IMAGES

GTX108356 WB Image

MCF-7 whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108356 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX108356 ICC/IF Image

HES1 antibody [N1], N-term detects HES1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HES1 protein stained by HES1 antibody [N1], N-term (GTX108356) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX108356 IP Image

HES1 antibody immunoprecipitates HES1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of HES1 by 2.5 μg of HES1 antibody (GTX108356) 12% SDS-PAGE The immunoprecipitated HES1 protein was detected by HES1 antibody (GTX108356) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX108356 WB Image

HES1 antibody [N1], N-term detects HES1 protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
12% SDS-PAGE
HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.