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HEXIM2 antibody

Anti-HEXIM2 antibody used in Western Blot (WB). GTX121524
Anti-HEXIM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121524
Anti-HEXIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX121524

Cat. No. GTX121524

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HEXIM2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

A549 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HEXIM2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HEXIM P-TEFb complex subunit 2 , L3

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-HEXIM2 antibody used in Western Blot (WB). GTX121524

GTX121524 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: HepG2
12% SDS PAGE
GTX121524 diluted at 1:500

Anti-HEXIM2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121524

GTX121524 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HEXIM2(GTX121524) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-HEXIM2 antibody used in IHC (Paraffin sections) (IHC-P). GTX121524

GTX121524 IHC-P Image

HEXIM2 antibody detects HEXIM2 protein at nucleus on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
HEXIM2 antibody (GTX121524) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169