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HFE antibody [3F1]

Anti-HFE antibody [3F1] used in Western Blot (WB). GTX60503
Anti-HFE antibody [3F1] used in Western Blot (WB). GTX60503
Anti-HFE antibody [3F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60503
Anti-HFE antibody [3F1] used in ELISA (ELISA). GTX60503

Cat. No. GTX60503

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3F1

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all HFE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HFE expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homeostatic iron regulator , HFE1 , HH , HLA-H , MVCD7 , TFQTL2

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HFE antibody [3F1] used in Western Blot (WB). GTX60503

GTX60503 WB Image

WB analysis of human HFE (AA: 125-282) recombinant protein using GTX60503 HFE antibody [3F1].

Anti-HFE antibody [3F1] used in Western Blot (WB). GTX60503

GTX60503 WB Image

WB analysis of HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate using GTX60503 HFE antibody [3F1].

Anti-HFE antibody [3F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60503

GTX60503 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60503 HFE antibody [3F1].
Green : HFE
Blue: DRAQ5 fluorescent DNA dye

Anti-HFE antibody [3F1] used in ELISA (ELISA). GTX60503

GTX60503 ELISA Image

ELISA analysis of antigen using GTX60503 HFE antibody [3F1].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409