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HLA-A antibody

Anti-HLA-A antibody used in Western Blot (WB). GTX54099
Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099
Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099
Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099

Cat. No. GTX54099

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all HLA-A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-285 of human HLA-A (NP_001229687.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class I, A , HLAA

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-A antibody used in Western Blot (WB). GTX54099

GTX54099 WB Image

WB analysis of various cell lysates using GTX54099 HLA-A antibody.
Loading : 25 μg
Dilution : 1:1000

Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099

GTX54099 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX54099 HLA-A antibody.
Dilution : 1:100

Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099

GTX54099 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX54099 HLA-A antibody.
Dilution : 1:100

Anti-HLA-A antibody used in IHC (Paraffin sections) (IHC-P). GTX54099

GTX54099 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX54099 HLA-A antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399