GeneTex
United States (US)

HLA-ABC antibody [W6/32]

HLA-A-HLA-B-HLA-C-antibody-W632_FACS_GTX27855-1_18121411_607.jpg

Cat No. GTX27855

Host Mouse
Clonality Monoclonal
Clone Name W6/32
Isotype IgG2a
Application ICC/IF, IHC-Fr, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

For IHC(frozen) a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

This antibody reacts with a monomorphic epitope of the 45 kD polypeptide, constiituting the HLAA,-B and-C or class I antigen of the human major histocompatibility complex.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The cell membranes of human tonsil lymphocytes

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HLA-F, HLA-A, HLAC, HLA-C, HLAA, HLAF, HLAG, major histocompatibility complex class I, HLAA, HLAB + HLAC, HLA-G, FLJ26655, AS, D6S204, HLA-B27, FLJ27082, HLA-B73, HLA-Cw, HLAB, HLA-JY3, MGC111087, HLC-C, SPDA1, PSORS1, HLA F, HLA A, HLA C, HLA G, HLC C, H

Cellular Localization

Cell membrane

Background

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.
DATA IMAGES
HLA-A-HLA-B-HLA-C-antibody-W632_FACS_GTX27855-1_18121411_607.jpg

GTX27855 FACS Image

Staining of peripheral blood lymphocytes using HLA-ABC antibody (GTX27855).

Package List Price ($)
$ 319