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HLA Class I antibody [W6/32]

Anti-HLA Class I antibody [W6/32] used in Flow cytometry (FACS). GTX27855

Cat. No. GTX27855

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 W6/32

Isotype

 IgG2a

Application

 ICC/IF, IHC-Fr, FACS, ELISA

Reactivity

 Human
Package
500 μg ($359)
See all HLA Class I products

APPLICATION

Application Note

For IHC(frozen) a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.

Product Note

This antibody reacts with a monomorphic epitope of the 45 kD polypeptide, constiituting the HLAA,-B and-C or class I antigen of the human major histocompatibility complex.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The cell membranes of human tonsil lymphocytes

Purification

Protein G affinity purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLA-F , HLA-A , HLAC , HLA-C , HLAA , HLAF , HLAG , major histocompatibility complex class I , HLAA , HLAB + HLAC , HLA-G , FLJ26655 , AS , D6S204 , HLA-B27 , FLJ27082 , HLA-B73 , HLA-Cw , HLAB , HLA-JY3 , MGC111087 , HLC-C , SPDA1 , PSORS1 , HLA F , HLA A , HLA C , HLA G , HLC C , H

Cellular Localization

Cell membrane

Background

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Research Area

DATA IMAGES

Anti-HLA Class I antibody [W6/32] used in Flow cytometry (FACS). GTX27855

GTX27855 FACS Image

Staining of peripheral blood lymphocytes using HLA Class I antibody (GTX27855).

REFERENCE

Clone Reference

Sekijima M et al. Xenotransplantation 2019;:e12543 Preparation of hybrid porcine thymus containing non-human primate thymic epithelial cells in miniature swine.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 359