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HLA-DOA antibody [N1C3]

Cat. No. GTX114470

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HLA-DOA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human HLA-DOA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620047

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major histocompatibility complex, class II, DO alpha , HLA-DNA , HLA-DZA , HLADZ

Cellular Localization

Membrane

Background

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq]

Database

Research Area

REFERENCE

Application Reference

Ningappa M et al. Am J Transplant 2016; 16 (2) : 497-508 Enhanced B Cell Alloantigen Presentation and Its Epigenetic Dysregulation in Liver Transplant Rejection.
Application : FACS
Reactivity : Human
Yoon T et al. Proc Natl Acad Sci U S A 2012; 109 (28) : 11276-81 Mapping the HLA-DO/HLA-DM complex by FRET and mutagenesis.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169