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HLA-G antibody [MEM-G/9] (Biotin)

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Western Blot (WB). GTX78333
Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Flow cytometry (FACS). GTX78333

Cat. No. GTX78333

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-G/9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all HLA-G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003).
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Purification

Purified IgG

Conjugation

Biotin
The reagent is free of unconjugated biotin.

RRID

AB_625687

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HLAG , MHCG , major histocompatibility complex, class I, G , HLA-G

Cellular Localization

Cell membrane,Secreted

Background

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Western Blot (WB). GTX78333

GTX78333 WB Image

Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) (GTX78332)and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).

Anti-HLA-G antibody [MEM-G/9] (Biotin) used in Flow cytometry (FACS). GTX78333

GTX78333 FACS Image

Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.

REFERENCE

Clone Reference

Lin YC et al. iScience 2023; 26 (3):106089 CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399