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HMG14 (phospho Ser20/Ser24) antibody

Anti-HMG14 (phospho Ser20/Ser24) antibody used in Western Blot (WB). GTX85600
Anti-HMG14 (phospho Ser20/Ser24) antibody used in IHC (Paraffin sections) (IHC-P). GTX85600

Cat. No. GTX85600

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
50 μg ($449)
See all HMG14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 20 μg/ml
ELISA 1:5000 - 1:25000

Note :

IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide from human HMGN1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

, HMG14 , HMGN1 , high mobility group nucleosome binding domain 1

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-HMG14 (phospho Ser20/Ser24) antibody used in Western Blot (WB). GTX85600

GTX85600 WB Image

WB analysis of various sample lysates using GTX85600 HMG14 (phospho Ser20/Ser24) antibody. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues.
Lane 1 : HMGN1, non-phosphorylated
Lane 2 : HMGN1, phosphorylated
Lane 3 : HMGN1 delta20E, delta24E, non-phosphorylated
Lane 4 : HMGN1, delta20E, delta24E, phosphorylated
Lana 5 : HMGN2, non-phosphorylated
Lane 6 : HMGN2, phosphorylated
Dilution : 1:1000

Anti-HMG14 (phospho Ser20/Ser24) antibody used in IHC (Paraffin sections) (IHC-P). GTX85600

GTX85600 IHC-P Image

IHC-P analysis of human skin tissue using GTX85600 HMG14 (phospho Ser20/Ser24) antibody.
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 20 μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449