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HMGB1 antibody [Giby-1-4]

Anti-HMGB1 antibody [Giby-1-4] used in (). GTX33926
Anti-HMGB1 antibody [Giby-1-4] used in Western Blot (WB). GTX33926

Cat. No. GTX33926

Host

 Human

Clonality

 Monoclonal

Clone Name

 Giby-1-4

Isotype

 IgG2

Application

 WB, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all HMGB1 products

APPLICATION

Application Note

Western blot: 1:1000

Calculated MW

25 kDa. ( Note )

Product Note

Recognizes human, mouse and rat HMGB1.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant HMGB1.

Purification

Protein A-affinity purified.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1

Cellular Localization

Nucleus , Cytoplasm , Secreted , Cell membrane , Endosome

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [Giby-1-4] used in (). GTX33926

GTX33926 Image

Anti-HMGB1 antibody [Giby-1-4] used in Western Blot (WB). GTX33926

GTX33926 WB Image

Western blot analysis of human and rat HMGB1 using anti-HMGB1, mAb (rec.) (GIBY-1-4) Different amounts of cell extracts from HEK293T cells (3μg, 5μg and 30μg) either transfected with a plasmid coding for rat HMGB1 (lanes 1, 2, 3) or non-transfected (lanes 4, 5, 6), were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-HMGB1,mAb (rec.) (GIBY-1-4) (1μg /ml). Proteins were visualized by a chemiluminescence detection system.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399