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HMGB1 antibody [J2E1]

Anti-HMGB1 antibody [J2E1] used in Western Blot (WB). GTX57549
Anti-HMGB1 antibody [J2E1] used in IHC (Paraffin sections) (IHC-P). GTX57549

Cat. No. GTX57549

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 J2E1

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all HMGB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone J2E1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HMGB3 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

high mobility group box 1 , HMG1 , HMG3 , HMGB1 , SBP1

Cellular Localization

Nucleus,Chromosome,Cytoplasm,Secreted,Cell membrane,Endosome,Endoplasmic reticulum-Golgi intermediate compartment

Background

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-HMGB1 antibody [J2E1] used in Western Blot (WB). GTX57549

GTX57549 WB Image

WB analysis of various samples using GTX57549 HMGB1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : MCF-7 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-HMGB1 antibody [J2E1] used in IHC (Paraffin sections) (IHC-P). GTX57549

GTX57549 IHC-P Image

IHC-P analysis of human colon cancer using GTX57549 HMGB1 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399