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HMGCS1 antibody

Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346
Anti-HMGCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112346
Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346
Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346
Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346

Cat. No. GTX112346

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all HMGCS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11179696

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA synthase 1 , HMGCS

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346

GTX112346 IHC-P Image

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGCS1 antibody (GTX112346) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGCS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112346

GTX112346 ICC/IF Image

HMGCS1 antibody detects HMGCS1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HMGCS1 protein stained by HMGCS1 antibody (GTX112346) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346

GTX112346 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (GTX112346) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-HMGCS1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112346

GTX112346 IHC-P Image

HMGCS1 antibody detects HMGCS1 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
HMGCS1 antibody (GTX112346) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HMGCS1 antibody used in Western Blot (WB). GTX112346

GTX112346 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (GTX112346) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

R Nakahara et al. EMBO J 2023; Hypoxia activates SREBP2 through Golgi disassembly in bone marrow-derived monocytes for enhanced tumor growth
Application : IHC-Fr
Han B et al. Mol Ther 2021; (Epub) A chemokine regulatory loop induces cholesterol synthesis in lung-colonizing triple-negative breast cancer cells to fuel metastatic growth.
Application : IHC-P
Reactivity : Human
Wang IH et al. Cancers (Basel) 2020; 12 (5) Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression.
Application : WB
Reactivity : Human
Lupo MG et al. Nutrients 2020; 12 (2) Cholesterol-Lowering Action of a Novel Nutraceutical Combination in Uremic Rats: Insights into the Molecular Mechanism in a Hepatoma Cell Line.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169