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HMGCS2 antibody

Anti-HMGCS2 antibody used in Western Blot (WB). GTX128013
Anti-HMGCS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128013

Cat. No. GTX128013

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HMGCS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

human HMGCS2-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885675

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxy-3-methylglutaryl-CoA synthase 2

Cellular Localization

Mitochondrion

Background

Mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase (HMGCS2; EC 2.3.3.10) mediates the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for brain, heart, kidney, and other organs during times of carbohydrate deprivation such as fasting (Robinson and Williamson, 1980 [PubMed 6986618]). Also see cytoplasmic HMG-CoA synthase (HMGCS1; MIM 142940), which mediates an early step in cholesterol synthesis.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-HMGCS2 antibody used in Western Blot (WB). GTX128013

GTX128013 WB Image

HMGCS2 antibody detects HMGCS2 protein by Western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 whole cell lysate/extract of human HMGCS2-transfected 293T cells
7.5 % SDS-PAGE
HMGCS2 antibody (GTX128013) dilution: 1:5000

Anti-HMGCS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX128013

GTX128013 IHC-P Image

HMGCS2 antibody detects HMGCS2 protein at mitochondria on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human liver carcinoma.
HMGCS2 antibody (GTX128013) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169