HNF6 antibody [4F12]

Cat. No. GTX83966

Host	Mouse
Clonality	Monoclonal
Clone Name	4F12
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all HNF6 products

Datasheet
Document

View product citations for antibody GTX83966 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 85-198 of human HNF6 (NP_004489) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

one cut homeobox 1 , HNF-6 , HNF6 , HNF6A

Cellular Localization

Nucleus

Background

Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.

Database

Gene ID: 3175 ONECUT1
UniProt: Q9UBC0 ONECUT1

Research Area

DATA IMAGES

GTX83966 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with HNF6 plasmid using GTX83966 HNF6 antibody [ 4F12].

GTX83966 IHC-P Image

IHC-P analysis of human liver carcinoma tissue using GTX83966 HNF6 antibody [ 4F12].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83966 WB Image

WB analysis of HEK293T cells transfected with HNF6 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83966 HNF6 antibody [ 4F12].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty