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HPD antibody [N1C2]

KO/KD Orthogonal
Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994
Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994
Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994

Cat. No. GTX103994

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HPD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HPD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950509

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

4-hydroxyphenylpyruvate dioxygenase , 4-HPPD , 4HPPD , GLOD3 , HPPDASE , PPD

Background

The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994

GTX103994 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HPD antibody [N1C2] (GTX103994) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994

GTX103994 WB Image

KO/KD

The data was published in the journal Cell Death Dis in 2019.PMID: 31285420

Anti-HPD antibody [N1C2] used in Western Blot (WB). GTX103994

GTX103994 WB Image

The data was published in the journal Cell Death Dis in 2019.PMID: 31285420

REFERENCE

Application Reference

Shan C et al. Cell Death Dis 2019; 10 (7) : 525 4-hydroxyphenylpyruvate dioxygenase promotes lung cancer growth via pentose phosphate pathway (PPP) flux mediated by LKB1-AMPK/HDAC10/G6PD axis.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169