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HSD17B8 antibody

Anti-HSD17B8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66143
Anti-HSD17B8 antibody used in Western Blot (WB). GTX66143

Cat. No. GTX66143

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all HSD17B8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B8 (NP_055049.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hydroxysteroid 17-beta dehydrogenase 8 , D6S2245E , FABG , FABGL , H2-KE6 , HKE6 , KE6 , RING2 , SDR30C1 , dJ1033B10.9

Cellular Localization

Mitochondrion matrix

Background

In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HSD17B8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66143

GTX66143 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX66143 HSD17B8 antibody.
Blue : DAPI
Dilution : 1:100

Anti-HSD17B8 antibody used in Western Blot (WB). GTX66143

GTX66143 WB Image

WB analysis of various sample lysates using GTX66143 HSD17B8 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399